Transcriptional regulation of the cinnamyl alcohol dehydrogenase gene from sweetpotato in response to plant developmental stage and environmental stress. Cinnamyl alcohol dehydrogenase (EC 1. Lignification is a tightly regulated and dynamic process subject to modulation at different levels during normal development and in response to different stresses (Boudet 2. In this study, we isolated the CAD c. DNA (Ib. CAD1) from sweetpotato, which can be phylogenetically categorized as belonging to the family of defense- related CADs. The Gly 2. 99 residue in Ib. CAD1 is a key determinant of substrate specificity and is a sinapaldehyde- specific residue according to Bomati and Noel (2. ![]() These researchers reported that the Gly residue at the base of the active site of Pt. SAD and Fxa. CAD is responsible for the high specificity of these proteins for the substrate sinapaldehyde. Pt. SAD is required for the biosynthesis of syringyl lignin (Li et al. Classical CAD enzymes involved in developmental lignification, such as At. Research Article : Use of Flyash in Agriculture: A Way to Improve Soil Fertility and its Productivity. ABSTRACTEvaluation of rice husk (RH) as bulking agent in bioremediation of automobile gas oil (AGO) hydrocarbon polluted agricultural soil using renewal by enhanced. CAD4, At. CAD5, and Eg. CAD2, have Phe instead of Gly in the active site for substrate specificity. At. CAD5 is able to use all substrates effectively, while At. CAD4 shows a very poor affinity for sinapaldehyde (Kim et al. Thus, Ib. CAD1 may have high affinity for the substrate sinapaldehyde based on its structure and, consequently, be involved in the defense- related process. Angiosperm CAD is encoded by a multigenic family consisting of members thought to have distinct roles, while gymnosperm CAD is encoded by a single gene (Mac. Kay et al. 1. 99. Zinser et al. 1. 99. Different CAD genes in angiosperms may be expressed in different tissues or at different stages during growth and development (Kim et al. Barakat et al. 2. Several authors have suggested that the chemical composition of defense lignin is different to that of developmental lignin. For example, syringyl lignin is accumulated in the plant cell wall during the hypersensitive resistance response in wheat (Menden et al. CAD activity is activated in F. ![]() ![]() Soil enzymatic activities and microbial biomass carbon (C mic) are considered to be two important soil biological activities influenced by oil contamination occurring. Hano et al. 2. 00. While much is known on how CAD is induced by biotic stress, such as fungal elicitor (Dixon and Paiva 1. Brill et al. 1. 99. CAD in relation to lignification under conditions of abiotic stress. We have investigated the transcriptional regulation of the Ib. CAD1 gene in response to various stresses. RT- PCR analysis shows that Ib. CAD1 gene expression in sweetpotato was strongly induced by both abiotic stress (cold) and biotic stress (wounding), including ROS (MV and H2. O2). ROS is a common signal that acts as a trigger of the downstream stress response. Fujita et al. (2. ROS may mediate crosstalk between biotic and abiotic stress- responsive gene- expression networks. Our RT- PCR results are consistent with those of our promoter analysis of Ib. CAD1. The activity of the Ib. CAD1 promoter was induced by the cold and H2. O2 treatments in this study, but the stress response was quite different in different tissues; for example, at the seedling stage, the cotyledons were more sensitive than the roots to Ib. CAD1 promoter induction. Among the stressors tested, wounding was found to be the strongest inducer of Ib. CAD1, and the wounding response was highly localized. These data suggest that the expression of the Ib. CAD1 gene is regulated in a tissue- specific manner under conditions of biotic and abiotic stresses. Hormone signaling pathways regulated by ABA, SA, JA, and ethylene as well as the ROS signaling pathway all play key roles in the crosstalk between biotic and abiotic stress responses (Fujita et al. ABA is extensively involved in the plant’s response to abiotic stresses, such as drought, low temperature and osmotic stress, and also regulates a variety of growth and developmental processes. In contrast, SA and JA play central roles in biotic stress signaling following pathogen infection and wounding. We identified several hormone- responsive cis- regulatory elements in the Ib. CAD1 promoter region, such as ABRE (ABA) (Yamaguchi- Shinozaki and Shinozaki 2. ARR binding site (cytokinin) (Yokoyama et al. SA) (Niggeweg et al. MYB responsive element, MYC responsive element (Yamaguchi- Shinozaki and Shinozaki 2. GARE (gibberellin) (Sutoh and Yamaguchi 2. At. MYC2, which was identified as an activator involved in the ABA- mediated drought stress signaling pathway, upregulates the expression of genes that are involved in the JA- mediated wounding response stress signaling pathway (Abe et al. Boter et al. 2. 00. Lorenzo et al. 2. Transgenic plants that overexpress both At. MYC2 and At. MYB2 show a greater sensitivity to ABA and enhanced osmotic stress tolerance (Abe et al. When we tested the GUS activity driven by the Ib. CAD1 promoter, we found that Ib. CAD1 promoter activity was enhanced when the plants were treated with various plant hormones, being most strongly induced by JA and SA, respectively. The Ib. CAD1 promoter was also induced by ABA and cytokinin (BA), but to a lesser degree. Taken together, these results indicate that Ib. CAD1 may function through a JA- and SA- mediated wounding response and an ABA- mediated cold response. The expression of the Ib. CAD1 gene was regulated during growth and development in a tissue- specific manner. Our histochemical analysis showed that weak GUS expression was observed in the xylem, phloem, and fiber of stems, and in the midrib and veins of mature leaves, which are lignified tissues of the plant vascular system. However, the Ib. CAD1 gene was strongly expressed in the area of the initiation sites for lateral roots developing from primary roots as well as in root tips—but not in the vascular cylinder of lignified root tissue. It has been reported that there is no lignification in root meristem cells, which is primarily involved in the constant progression of cell division (de Obeso et al. GUS expression was also strong in anther and pollen. We have previously shown that Ib. CAD1 is the most abundant gene in the EST library of cells grown in suspension culture and that it is highly expressed at the actively dividing- log phase. In light of these results, the expression of the Ib. CAD1 promoter indicates a new role of CAD protein in lateral root formation, cell- wall synthesis, and the promotion of cell division that is unrelated to constitutive lignin formation. We suggest that Ib. CAD1 may also be involved in lignifications induced by both abiotic and abiotic stresses and in tissue- specific developmental lignification. Further analysis of the Ib. CAD1 gene will provide an insight into the signaling pathway involved in the regulation of biotic and abiotic stresses in lignin biosynthesis as well as into the role of CAD protein during cell division. A Way to Improve Soil Fertility and its Productivity. Adriano, D. C., A. L. Page, A. A. Elseewi, A. C. Chang and I. Straugham, 1. Utilization and disposal of fly- ash and coal residues in terrestrial ecosystem: A review. J. Environ. Qual., 9: 3. Direct Link | Adriano, D. C., T. A. Woodford and T. G. Ciravolo, 1. 97. Growth and elemental composition of corn and bean seedlings as influenced by soil application of coal ash. J. Environ. Qual., 7: 4. Direct Link | Aitken, R. L., D. J. Campbell and L. C. Bell, 1. 98. 4. Properties of Australian fly ash relevant to their agronomic utilization. Aust. J. Soil Res., 2. Direct Link | Buck, J. K., R. J. Honston and W. A. Beimborn, 1. 99. Direct seedling of anthracite refuge using coal flyash as a major soil amendment. Proceedings of the Mining and Reclamation Conference and Exhibition, April 2. Charleston, West Virginia, pp: 2. Capp, J. P., 1. 97. Power Plant Flyash Utilization for Land Reclamation in the Eastern United States. In: Reclamation of Drastically Disturbed Lands, Schaller, F. W. and P. Sutton (Eds.). Madison, WI., ASA., pp: 3. Carlson, C. L. and D. C. Adriano, 1. 99. Environmental impacts of coal combustion residues. J. Environ. Qual., 2. Cross. Ref | Direct Link | Cerevelli, S., G. Petruzzelli, A. Perna and R. Menicagli, 1. 98. Soil nitrogen and fly ash utilization: A laboratory investigation. Agrochemica, 3. 0: 2. Direct Link | Cetin, S. E. Pehlivan, 2. 00. The use of flyash as a low cost, environmentally friendly alternative to activated carbon for the removal of heavy metals from aqueous solutions. Colloids Surfaces A: Physicochem. Eng. Aspects, 2. 98: 8. Cross. Ref | Chang, A. C., L. J. Lund, A. L. Page and J. E. Warneke, 1. 97. 7. Physical properties of fly ash- amended soils. J. Environ. Qual., 6: 2. Cross. Ref | Direct Link | Elliott, L. F., D. Tittemore, R. L. Papendick, V. L. Cochran and D. F. Bezidicek, 1. 98. The effect of Mount St. Helen's ash on soil microbial respiration and numbers. J. Environ. Qual., 1. Direct Link | Ferreira, C., A. Ribeiro and L. Ottosen, 2. Possible applications for municipal solid waste fly ash. J. Hazard. Mater., 9. Cross. Ref | Finkelman, R. B., H. E. Belkin, B. S. Zhang and J. A. Centeno, 2. 00. 0. Arsenic poisoning caused by residential coal combustion in Guizhou Province, P. R. China. Proceedings of the 3. International Geological Congress, (IGC'0. Rio de Janeiro, Brazil, pp: 4. Furr, A. K., T. F. Parkinson, R. A. Hinrichs, D. R. Van Campen and C. A. Bache et al., 1. National survey of elements and radioactivity in fly ashes. Absorption of elements by cabbage grown in fly ash soil mixtures. Environ. Sci. Technol., 1. Direct Link | Gaind, S. A. C. Gaur, 2. 00. Evaluation of fly ash as a carrier for diazotrophs and phosphobacteria. Bioresour. Technol., 9. Cross. Ref | Garampalli, R. H., S. Deene and C. Narayana Reddy, 2. Infectivity and efficacy of Glomus aggregatum and growth response of Cajanus cajan (L.) Millsp. J. Environ. Biol., 2. Direct Link | Garau, M. A., J. L. Dalmau and M. T. Felipo, 1. 99. Nitrogen mineralization in soil amended with sewage sludge and fly ash. Biol. Fert. Soils, 1. Direct Link | Ghodrati, M., J. T. Sims and B. S. Vasilas, 1. 99. 5. Evaluation of flyash as a soil amendment for the Atlantic coastal plain. I. Soil hydraulic properties and elemental leaching. J. Water Soil Air Pollut., 8. Direct Link | Goyal, D., K. Kaur, R. Garg, V. Vijayan and S. K. Nanda et al., 2. 00. Industrial Fly Ash as a Soil Amendment Agent for Raising Forestry Plantations. In: Fundamental of Advanced Materials for Energy Conversion, Taylor, P. R. (Ed.). TMS Publication, Warrendale, PA, pp: 2. Grewal, K. S., P. S. Yadav, S. C. Mehta and M. C. Oswal, 2. 00. 1. Direct and residual effect of fly ash application to soil on crop yield and soil properties. Crop Res., 2. 1: 6. Gupta, A. K., S. Dwivedi, S. Sinha, R. D. Tripathi, U. N. Rai and S. N. Singh, 2. Metal accumulation and growth performance of Phaseolus vulgaris grown in fly ash amended soil. Bioresour. Technol., 9. Cross. Ref | Gupta, G. S., G. Prasad and V. H. Singh, 1. 99. 0. Removal of chrome dye from aqueous solutions by mixed adsorbents: Flyash and coal. Water Res., 2. 4: 4. Hall, J. L. and L. E. Williams, 2. 00. Transition metal transporters in plants. J. Exp. Bot., 5. 4: 2. Cross. Ref | Direct Link | Hodgson, D. R. and R. Holliday, 1. The agronomic properties of pulverized fuel ash. Chem. Ind., 2. 0: 7. Hrynkiewiez, K., C. Baum, J. Niedojadlo and H. Dahm, 2. 00. 9. Promotion of mycorrhiza formation and growth of willows by the bacterial strain Sphingomonas sp. L on fly ash. Biol. Fert. Soils, 4. 5: 3. Cross. Ref | Direct Link | Inam, A., 2. Use of flyash in turnip (Brassica rapa L.) cultivation. Pollut. Res., 2. 6: 3. Direct Link | Jala, S. D. Goyal, 2. 00. 6. Flyash as a soil ameliorant for improving crop production- a review. Bioresour. Technol., 9. Cross. Ref | Jamwal, N., 2. Is it all grey. Down Earth, 3. Kalra, N., H. C. Joshi, A. Chaudhary, R. Chaudhary and S. K. Sharma, 1. 99. Impact of fly ash incorporation in soil on germination of crops. Bioresour. Technol., 6. Direct Link | Kene, D. R., S. A. Lanjewar and B. M. Ingole, 1. 99. 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Chemosphere, 7. 2: 6. Direct Link | Lal, J. K., B. Mishra and A. K. Sarkar, 1. 99. Effect of fly ash on soil microbial and enzymatic activity. J. Ind. Soc. Soil Sci., 4. Lau, S. S. S. and J. W. C. Wong, 2. 00. Toxicity evaluation of weathered coal fly ash amended manure compost. Water, Air Soil Pollut., 1. Direct Link | Lee, H., H. S. Ha, C. H. Lee, Y. B. Lee and P. J. Kim, 2. Fly ash effect on improving soil properties and rice productivity in Korean paddy soils. Bioresour. Technol., 9. Cross. Ref | Direct Link | Machulla, G., S. Zikeli, M. Kaster and R. Jahn, 2. 00. 4. Microbial biomass and respiration in soils derived from lignite ashes: A profile study. J. Plant Nutr. Soil Sci., 1. Cross. Ref | Matsi, T. V. Z. Keramidas, 1. Flyash application on two acid soils and its effect on soil salinity, p. H, B, P and on ryegrass growth and composition. Environ. Pollut., 1. Direct Link | Matti, S. S., T. M. Mukhopadhyay, S. K. Gupta and S. K. Banerjee, 1. 99. 0. 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You can directly execute command line switches in the following way: Open the Run command by pressing the Windows Logo + R on your keyboard. Use command-line switches to customize the startup process for Microsott Office programs: Word, Excel, PowerPoint, Outlook, and Access. · After installing Windows Server 2008 Core you might want to disable the integrated firewall. You can perfom that by using the following command. Windows Server 2003, Windows Server 2003 SP1 and SP2, and Windows Server 2003 R2 retired content. The content you requested has already retired. It's available to. Microsoft Data Access Components (MDAC; also known as Windows DAC) is a framework of interrelated Microsoft technologies that allows programmers a uniform and. ![]() ![]() Failing this, as an apparent *nix fan, why don't you consider Linux + Mono? Use Power. Shell Invoke- Command to run scripts on remote computers – 4sysops. The Invoke- Command cmdlet is one way to leverage Power. Shell Remoting. In today’s post, I will give you an overview of Invoke- Command. Michael Pietroforte is the founder and editor of 4sysops. He is a Microsoft Most Valuable Professional (MVP) with more than 3. IT management and system administration. Latest posts by Michael Pietroforte (see all)Introducing Power. Shell Remoting ^When it comes to managing remote computers with Power. Shell, you have essentially three options. You can open an interactive session with the Enter- PSSession cmdlet (One- to- One Remoting). An alternative is the Invoke- Command cmdlet, which allows you to run remote commands on multiple computers (which is why it is called One- to- Many Remoting). PowerShell Remoting depends on Windows Remote Management (WinRM), which is Microsoft’s implementation of the WS-Management (WS-Man) protocol. MS16-148: Security Update for Microsoft Office to Address Remote Code Execution: December 13, 2016. Using Terminal Services and RemoteApp to Extend Your Microsoft Access and other Windows Applications Over the Internet.The third option is to use one of those cmdlets that offer a Computer. Name parameter. In most cases, Power. Shell Remoting isn’t involved then. To list those cmdlets, you can use this command. Get- Command - Parameter. Name Computer. Name. Get- Command- Parameter. Name Computer. Name. Cmdlets with the Computer. Name parameter. In addition to the built- in cmdlets, quite a few Power. Shell modules exist that support remote management. For instance, you can manage Active Directory from your workstation after installing the Active Directory module. Whenever one of these options exists, I would avoid enabling Power. Shell Remoting on the remote machine. Because Power. Shell Remoting presents a security risk, it has to be enabled even within an Active Directory domain. You can also use Remoting on workgroup computers, but you will have to take care of a few extra configurations. If you don’t configure Remoting properly, you will run into errors such as Win. RM cannot process the request or Access denied. In the latter case, you probably failed to provide admin credentials. Power. Shell Remoting depends on Windows Remote Management (Win. RM), which is Microsoft’s implementation of the WS- Management (WS- Man) protocol. The protocol relies on HTTP or HTTPS and uses the TCP ports 5. WS- Management encrypts all Power. Shell communication even if you only work with HTTP. However, this kind of encryption is vulnerable to man- in- the middle attacks. I therefore recommended to use HTTPS instead of HTTP in insecure networks. I will say more about this topic in a follow- up post. Executing cmdlets with Invoke- Command ^Perhaps the most interesting form of Power. Shell remote management is One- to- Many Remoting with the Invoke- Command cmdlet. This cmdlet allows you to execute Power. Shell commands on multiple remote computers that contain cmdlets that don’t feature the - Computer. Name parameter. Two ways exist to connect to remote computers with Invoke- Command. You usually use the ‑Computer. Name parameter to manage Windows machines. Alternatively, you can pass the ‑Connection. Uri parameter to manage backend applications, such as Exchange, or cloud services such as Azure. In the remainder of this article, I will focus on managing remote Windows machines that are Active Directory domain members. The command below executes the Get- Child. Item cmdlet on the machine whose computer name is stored in the $Remote. Computer variable. Invoke- Command - Computername $Remote. Computer - Script. Block { Get- Child. Item "C: \Program Files" }1. Invoke- Command- Computername$Remote. Computer- Script. Block{Get- Child. Item"C: \Program Files"}Power. Shell automatically creates a so- called PSSession on the remote computer, which essentially is a user- created Power. Shell session as opposed to a session that a Power. Shell host such as the Power. Shell console or Power. Shell ISE creates. The output of the executed commands in the script block is automatically relayed to your local session. As usual, you can interrupt the displaying of the output with CTRL+C. As with the Enter- PSSession cmdlet, you have to be an administrator on the remote machine. Otherwise, you will receive an Access denied error message. If you are not logged on as domain administrator on your local machine, you have to provide sufficient credentials with the - Credential parameter. You can pass multiple computers to the - Computer. Name parameter by separating their names with commas. If you also want to include the local computer, you add “localhost” or simply “.” to the list. To run multiple commands, you can separate them with semicolons in the script block. However, a better option is to use the - File. Path parameter instead of the - Script. Block parameter, which allows you to execute an entire Power. Shell script. Invoke- Command - Computer. Name PC1,PC2,PC3 - File. Path C: \my. Folder\my. Script. ps. 11. Invoke- Command- Computer. Name PC1,PC2,PC3- File. Path. C: \my. Folder\my. Script. ps. 1By default, Power. Shell will send the script immediately to all computers if 3. If more than 3. 2 computer names are passed, Power. Shell will queue the surplus computers until the script completes in one of the first 3. PSSessions. You can change this default behavior with the - Throttle. Limit parameter. However, you should be aware of the implications on the load of your computer and the network when the results come pouring in. Testing if Remoting is enabled ^In particular, if you plan to execute commands on many machines, you might want to test their availability with the Test- Connection cmdlet first. This cmdlet sends ICMP echo request packets (pings, that is) to the remote computers. If (Test- Connection - Computer. Name $Remote. Computers - Quiet). Invoke- Command - Computer. Name $Remote. Computers - Script. Block {Get- Child. Item “C: \Program Files”}. If(Test- Connection- Computer. Name$Remote. Computers- Quiet){ Invoke- Command- Computer. Name$Remote. Computers- Script. Block{Get- Child. Item“C: \Program Files”}}The - Quiet parameter suppresses the output of the cmdlet and returns $True if the remote machine answers the pings. This method is fine if you can be sure that Remoting is enabled on the remote machines. If you want a more bullet- proof test, you could use the Test- WSMan cmdlet to check whether the Win. RM service can be accessed on the remote machine. However, a downside of this method is that the cmdlet’s timeout can be several seconds and can’t be configured. If you have many computers in your list, this can slow down your control script on the local machine significantly. An alternative is to work with a try- catch block. Remote. Computers = @("PC1","PC2"). For. Each ($Computer in $Remote. Computers). Invoke- Command - Computer. Name $Computer - Script. Block {Get- Child. Item "C: \Program Files"} - Error. Action Stop. Add- Content Unavailable- Computers. Computer. }$Remote. Computers=@("PC1","PC2")For. Each($Computer in$Remote. Computers){ Try { Invoke- Command- Computer. Name$Computer- Script. Block{Get- Child. Item"C: \Program Files"}- Error. Action Stop } Catch { Add- Content Unavailable- Computers. Computer }}If Invoke- Command fails to execute the command on the computer, the corresponding computer name is added to a text file (line 1. This enables you to repeat the command later. Passing local variables to the remote session ^If you want to pass variables from your control script on the local computer to the commands that run on the remote computers, you can work with the - Argument. List parameter. $Name = "svchost". Invoke- Command –Computer. Name $Remote. Computer –Script. Block { param($RName,$RCPU) Get- Process - Name $RName | Where - Property CPU - gt $RCPU } - Argument. List $Name,$CPU$Name="svchost"$CPU=1. Invoke- Command–Computer. Name$Remote. Computer–Script. Block{param($RName,$RCPU)Get- Process- Name$RName|Where- Property. CPU- gt$RCPU}- Argument. List$Name,$CPUIn the example, the values of the $Name and $CPU variables are passed to the $RName and $RCPU variables, which the Get- Process cmdlet will use on the remote computer to determine all processes with the name “svchost” where the CPU usage (CPU property of Get- Process) is greater than 1. By the way, this example also demonstrates that Power. Shell Remoting is sometimes your only option even if the cmdlet you want to use offers a - Computer. Name parameter. The Get- Process cmdlet cannot retrieve the CPU usage from a remote computer if you run it in a local session. However, if you use the Invoke- Command cmdlet, Get- Process runs in a remote session and you can then read the CPU usage. Accessing methods in Power.
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